于放，男，教授，博士生导师。辽宁省教育厅生物科学类教指委委员，辽宁省学位委员会学科评议组成员，中国植物生理与植物分子生物学学会植物代谢专业委员会委员，中国野生植物保护协会科技委专家委员，辽宁省植物生理学会常务理事，《植物研究》核心学术期刊编委，《大连工业大学学报》核心学术期刊编委。1974年5月出生。2005年6月毕业于大连理工大学生物化工专业，博士学位。2005至2013年分别在中科院上海植物生理生态研究所、荷兰Leiden University以及加拿大Brock University从事科研工作，2014年3月至大连工业大学工作被评聘为教授。
1. 主要学术研究方向：
(1) 植物次生代谢途径的调控及解析：利用分子生物学及化学手段完成对植物次生代谢物的合成途径相关基因（代谢途径酶基因、转录因子、以及转运基因等）的克隆及鉴定，进而实现对代谢途径的有效调控。
(2) 植物代谢与现代农业：系统研究农业微生物代谢对植物抗性及生长的调节作用，进而揭示发酵产品作为生物农药/农肥调控植物生长的分子机理，为优化从发酵/生物转化法出发进而高效生产农药/农肥提供必需的理论基础。
(3) 生物催化及酶工程：通过基因挖掘及蛋白质工程获得医药、化学品以及天然产物等所需的重要酶，并通过对宿主细胞的驯化及改造，进而利用微生物发酵/生物转化实现对生物制品的高效生产。
2. 招收博士研究生所在学科：轻工技术与工程
3. 招收硕士研究生所在学科：轻工技术与工程；生物学；生物与医药（专硕）
4. 目前承担主要课程：分子生物学；高级细胞生物学
5. 发表论文情况：近年来在Science, PNAS, Current Opinion in Plant Biology, The
Plant Journal, Plant Physiology, Tree Physiology等著名学术期刊上发表多篇论文。
近年发表学术论文摘选：
(1) Chunhao Chang, Xiaorui
Guo, Biying Wang, Yanyan Wang, Mengxia Zhang, Fang Yu*, Zhonghua Tang*. CrGRF1/4
Mediating Light Signal to Regulate Monoterpenoid Indole Alkaloid Biosynthesis
In Catharanthus roseus, Plant Cell & Environment, 2025, https://doi.org/10.1111/pce.15484
(2) Xiaoxiao Gao, Xiaona
Zhu, Zhiqin Wang, Xuejing Liu, Rui Guo, Jing Luan, Zhiwen Liu, Fang Yu*.
Modulation of Terpenoid Indole Alkaloid Biosynthesis in Catharanthus roseus by Sphingomonas Sp Y503 via the CrMAPKKK1-CrMAPKK1/CrMAPKK2-CrMPK3
Signaling Cascade, Plant Cell & Environment, 2024, 48(2): 1692-1704. https://doi.org/10.1111/pce.15253
(3) Ping Kou, Yingying Yu,
He Wang, Yuchi Zhang, Zhaoxia Jin and Fang Yu *. An Integrated Strategy
Based on 10-DAB Extraction and In Situ Whole-Cell Biotransformation of
Renewable Taxus Needles to Produce Baccatin III, Molecules, 2024, 29(11):
2586. https://doi.org/10.3390/molecules29112586
(4) Liya Ma, Jia Yuan, Hao
Qin, Mengxia Zhang, Fengxia Zhang, Fang Yu, Zhixi Tian, and Guodong
Wang*. GmMATE100 Is Involved in the Import of Soyasaponins A and B into
Vacuoles in Soybean Plants (Glycine max L.). Journal of Agricultural and Food
Chemistry, 2024, 72(17): 9994-10004. https://doi.org/10.1021/acs.jafc.4c01774
(5) Yue Zhang, Changhao
Liu, Siqi Chen, Chengcheng Fan, Zhaoxia Jin, Fang Yu*. One-pot synthesis
and biochemical characterization of the novel magnetic phospholipase D
nanoflowers modified with polyphenols. Molecular Catalysis, 2024, 559: 114056. https://doi.org/10.1016/j.mcat.2024.114056
(6) Mengxia Zhang, Bingrun
Yang, Yanyan Wang and Fang Yu*. CrJAT1 Regulates Endogenous JA Signaling
for Modulating Monoterpenoid Indole Alkaloid Biosynthesis in Catharanthus
roseus. Genes, 2024, 15(3): 324. https://doi.org/10.3390/genes15030324
(7) Mengxia Zhang, Shiqi
Zhang, Bingrun Yang, Chunhao Chang, Zhiwen Liu, Fang Yu*. Feedback
regulation of salicylic acid signaling by isochorismate synthase1 modulates camptothecin
biosynthesis in Camptotheca acuminata. Plant Cell, Tissue and Organ
Culture, 2024, 156(3): 90. https://doi.org/10.1007/s11240-024-02711-x
(8) Yanyan Wang, Yang Wang,
AiKun Pan, Qi Miao, Yuqian Han, Zhiwen Liu, Fang Yu*. CaERF1- mediated
ABA signal positively regulates camptothecin biosynthesis by activating the
iridoid pathway in Camptotheca acuminata. International Journal of
Biological Macromolecules, 2024, 261: 129560. https://doi.org/10.1016/j.ijbiomac.2024.129560
(9) Yue Zhang*, Yu Gao,
Jiahui Chen, Fang Yu, Yongming Bao*. Overexpression and truncation of a
novel cold-adapted lipase with improved enzymatic characteristics. Protein
Expression and Purification, 2024, 214: 106376. https://doi.org/10.1016/j.pep.2023.106376.
(10) Jiancong Cao, Bingrun
Yang, Mengxia Zhang, Fang Yu*. Regulation of T16H subcellular
localization for promoting its catalytic efficiency in yeast cells.
Biotechnology Letters, 2023, 46(1): 29-35. https://doi.org/10.1007/s10529-023-03442-3
(11) Xiaoxiao Gao, Jing
Luan, Lifei Wang, Hui Li, Qinghua Wang, Zhiqin Wang, Zhaoxia Jin, Fang Yu*.
Effect of the Plant Growth Promoting Rhizobacterium, Cronobacter sp.
Y501, for Enhancing Drought Tolerance in Maize (Zea mays L.). Journal of
Soil Science and Plant Nutrition, 2023, 23(2): 2786-2797. https://doi.org/10.1007/s42729-023-01234-2
(12) Yanyan Wang, Yang
Wang, Hefei Bai, Yuqian Han and Fang Yu*. An ABCG-Type Transporter
Facilitates ABA Influx and Regulates Camptothecin Biosynthesis in Camptotheca
acuminata. International Journal of Molecular Sciences, 2022, 23(24): 16120.
https://doi.org/10.3390/ijms232416120
(13) Zhaoxia Jin*, Qiyao
Zhu, Yangyang Guo, Ronglian Xing, Yanyan Wang*, Yue Zhang, Xiaoxiao Gao, Fang
Yu*. Functional characterization of secologanin synthase-like homologous
genes suggests their involvement in the biosynthesis of diverse metabolites in
the secoiridoid biosynthetic pathway of Camptotheca acuminata Decne.
International Journal of Biological Macromolecules, 2022, 222: 2594-2602. https://doi.org/10.1016/j.ijbiomac.2022.10.042
(14) Hengwei Zhang, Ke Zhang,
Jiarui Liu, Yi Wang*, Fang Yu*. The sensing mechanism of fluorescent
probe for PhSH and the process of ESIPT. Photochemical and Photobiological
Sciences, 2022, 21: 1055-1065. https://doi.org/10.1007/s43630-022-00193-4
(15) Huihan Ma, Yu Zhao,
Zijie Lu, Ronglian Xing, Xuemei Yao, Zhaoxia Jin*, YanyanWang, Fang Yu*.
Citral-loaded chitosan/carboxymethyl cellulose copolymer hydrogel microspheres
with improved antimicrobial effects for plant protection. International Journal
of Biological Macromolecules, 2020, 164: 986-993. https://doi.org/10.1016/j.ijbiomac.2020.07.164
(16) Yanyan Wang, Bingrun
Yang, Mengxia Zhang, Shanshan Jia, Fang Yu*. Application of transport
engineering to promote catharanthine production in Catharanthus roseus hairy roots. Plant Cell, Tissue and Organ Culture, 2019, 139: 523-530. https://doi.org/10.1007/s11240-019-01696-2
(17) Zhaoxia Jin*, Yunhan
Cong, Suliang Zhu, Ronglian Xing, Dawei Zhang, Xuemei Yao, RuyiWan, Yanyan
Wang, Fang Yu*. Two classes of cytochrome P450 reductase genes and their
divergent functions in Camptotheca acuminata Decne, International
Journal of Biological Macromolecules, 2019, 138: 1098-1108. https://doi.org/10.1016/j.ijbiomac.2019.07.141
(18) Zhaoxia Jin*, Ruyi
Wan, Ruxue Yan, Yingying Su, Honglan Huang, Lihan Zi, Fang Yu*. Microwave
assisted extraction of multiple trace levels of intermediate metabolites for
camptothecin biosynthesis in Camptotheca acuminata and their
simultaneous determination by HPLC-LTQ-orbitrap-MS/MS and HPLC-TSQ-MS, Molecules,
2019, 24(4): 815. https://doi.org/10.3390/molecules24040815
(19) Chunhao Chang, Zhiwen
Liu, Yanyan Wang, Zhonghua Tang, Fang Yu*. A bZIP transcription factor,
CaLMF, mediated light-regulated camptothecin biosynthesis in Camptotheca
acuminata, Tree Physiology, 2019, 39(3): 372-380. https://doi.org/10.1093/treephys/tpy106
(20) Zhiwen Liu, Qiong Wu,
Ya Li, Shi Liu, Lin Li, Qiujun Chen, Yanyan Wang, Fang Yu*. Effects of
exogenous salicylic acid on accumulation of camptothecin and gene expression in Camptotheca acuminata, Canadian Journal of Forest Research, 2019, 49(1):
104-110. https://doi.org/10.1139/cjfr-2018-0260
(21) Ya-jie Pan, Ying-chao
Lin, Bo-fan Yu, Yuan-gang Zu, Fang Yu* and Zhong-Hua Tang*. Transcriptomics
comparison reveals the diversity of ethylene and methyl-jasmonate in roles of
TIA metabolism in Catharanthus roseus, BMC genomics, 2018, 19: 508. https://doi.org/10.1186/s12864-018-4879-3
(22) Yu Zhao, Yinfeng Xu, Fang
Yu*, and Chunzhi Zhang*. Identification of a novel phospholipase D gene and
effects of carbon sources on its expression in Bacillus cereus ZY12,
Journal of Microbiology, 2018, 56: 264-271. https://doi.org/10.1007/s12275-018-7529-1
(23) Yanyan Wang, Ye Wang, Song An, Jinrui Zhang, Yuqian Han, Jinge Xu, Fang
Yu, Dahai Yu and Xuexun Fang* Potent and selective inhibition of matrix
metalloproteinases by lanthanide trichloride, RSC Advances, 2018, 8: 14347. https://doi.org/10.1039/C8RA00871J
(24) Zerihun Demessie, Kathlyn N. Woolfson, Fang Yu, Yang Qu,
Vincenzo De Luca*. The ATP binding cassette transporter, VmTPT2/VmABCG1, is
involved in export of the monoterpenoid indole alkaloid, vincamine in Vinca
minor leaves, Phytochemistry, 2017, 140: 118-124. https://doi.org/10.1016/j.phytochem.2017.04.019
(25) Zhaoxia Jin*, Lin Gao, Lin Zhang, Tianyi Liu, Fang Yu,
Zongshen Zhang, Qiong Guo & Biying Wang. Antimicrobial activity of saponins
produced by two novel endophytic fungi from Panax notoginseng, Natural
Product Research, 2017, 31(22): 2700-2703. https://doi.org/10.1080/14786419.2017.1292265
(26) Zhaoxia Jin*, Dandan Li, Tianyi Liu, Fang Yu, Zongshen
Zhang, Caixia Su, Yanyan Wang, Qiong Guo and Zhiwen Liu. Cultural endophytic
fungi associated with Dendrobium officinale: identification, diversity
estimation and their antimicrobial potential, Current Science, 2017, 112: 25. https://www.jstor.org/stable/44211901
(27) Zhaoxiao Jin, Tongshuai Yan1, Chunhao Chang, Zhiwen Liu, Yanyan
Wang, Zhonghua Tang*, Fang Yu*. Application of virus-induced gene
silencing approach in Camptotheca acuminata, Plant Cell, Tissue and
Organ Culture, 2016, 126(3): 533-540. https://doi.org/10.1007/s11240-016-1022-5
(28) Dylan Levac, Paulo Cázares, Fang Yu, Vincenzo De Luca*. A
Picrinine N-Methyltransferase Belongs to a New Family of
γ-Tocopherol-Like Methyltransferases Found in Medicinal Plants That Make
Biologically Active Monoterpenoid Indole Alkaloids, Plant Physiology，2016, 170(4): 1935-1944. https://doi.org/10.1104/pp.15.01813
(29) Liu Zhiwen*, Zhang Shiqi, Zhai Dong, Yu Fang, Jin Zhaoxia,
Chen Wenfu*. Molecular phylogeny of the Chinese weedy rice based on nrDNA ITS
and two cpDNA sequences, INDIAN JOURNAL OF GENETICS AND PLANT BREEDING, 2016,
76(01): 57-63. https://doi.org/10.5958/0975-6906.2016.00008.0
(30) Siriguleng Qian∗, Chun Wang, Hongying Wang, Fang
Yu, Chunzhi Zhang, Hongshan Yu. Synthesis and characterization of
surface-functionalized paramagnetic nanoparticles and their application to
immobilization of α-acetolactate decarboxylase, Process Biochemistry, 2015, 50(9):
1388-1393. https://doi.org/10.1016/j.procbio.2015.05.012
(31) Vincenzo De Luca*, Vonny Salim, Antje Thamm, Sayaka Atsumi Masada
and Fang Yu. Making iridoids/secoiridoids and indole alkaloids: Progress
on pathway elucidation, Current Opinion in Plant Biology, 2014, 19: 35-42. https://doi.org/10.1016/j.pbi.2014.03.006
(32) Vonny Salim, Brent Wiens, Sayaka Masada-Atsumi, Fang Yu,
Vincenzo De Luca*. 7-Deoxyloganetic acid synthase catalyzes a key 3 step oxidation to form
7-deoxyloganetic acid in Catharanthus roseus iridoid biosynthesis,
Phytochemistry, 2014, 101: 23-31. https://doi.org/10.1016/j.phytochem.2014.02.009
(33) Fang Yu and Vincenzo De Luca*. ATP-binding cassette
transporter controls leaf surface secretion of anticancer drug components in Catharanthus
roseus, Proc. Natl. Acad. Sci. U.S.A. 2013, 110(39): 15830-15835. https://doi.org/10.1073/pnas.1307504110
(34) Fang Yu, Antje M.K. Thamma, Darwin Reed, Nemesio
Villa-Ruano, Alfonso Lara Quesada, Edmundo Lozoya Gloria, Patrick Covello,
Vincenzo De Luca*. Functional characterization of amyrin synthase involved in
ursolic acid biosynthesis in Catharanthus roseus leaf epidermis,
Phytochemistry, 2013, 91: 122-127. https://doi.org/10.1016/j.phytochem.2012.05.002
(35) Vonny Salim, Fang Yu, Joaquin Altarejos and Vincenzo De Luca*.
Virus induced gene silencing identifies Catharanthus roseus 7-deoxyloganic
acid-7-hydroxylase, a step in iridoid and monoterpene indole alkaloid
biosynthesis, The Plant Journal, 2013, 76(5): 754-765. https://doi.org/10.1111/tpj.12330
(36) Vincenzo De Luca*, Vonny Salim, Sayaka Masada Atsumi, Fang Yu.
Mining the biodiversity of plants: a revolution in the making, Science, 2012,
336(6089): 1658-1661. https://doi.org/10.1126/science.121741
6. 承担科研项目情况：主持及参与多项国家及省科研项目，承担的主要科研项目：
(1) 科技部关于科技基础资源调查专项：东北禁伐林区野生经济植物资源调查（2019-2024），子课题，主持；
(2) 国家自然科学基金面上项目：喜树叶表皮细胞中环烯醚萜途径响应水杨酸信号动态调控喜树碱生物合成的分子机制 (2020-2023)，主持；
(3) 辽宁省自然基金资助计划：CaLMF介导光信号调控喜树碱生物合成的分子机制 (2019-2022)，主持；
(4) 国家自然科学基金面上项目：长春花单萜吲哚生物碱生物合成途径中代谢物的胞间转运机制及代谢流调控初探 (2016-2019)，主持；
(5) 辽宁省高等学校创新人才支持计划 (2017-2019)，主持；
(6) 辽宁省教育厅重点领域攻关专项：药用植物次生代谢途径快速解析体系的建立 (2016-2018)，主持；
(7) 辽宁省自然科学基金面上项目：长春碱合成途径代谢流通量调控及中间代谢产物转运基因的克隆 (2015-2016)，主持；
(8) 国家自然科学基金面上项目：共表达分析及内生菌转录组比对结合VIGS技术实现喜树碱合成途径的快速解析 (2017-2020)，参与；
(9) 辽宁省自然科学基金面上项目：不同诱抗模式下ABC Transporter 介导植物根系分泌物外排参与根际微生物差异性应答响应机制 (2018-2020)，参与；
(10) 辽宁省自然科学基金面上项目：转录因子CaHY5在喜树碱生物合成及信号转导中的功能分析 (2015-2016)，参与；
(11) 横向项目：生物酶高效催化生产β-丙氨酸 (2017-2019)，参与。
7. 出版著作情况：撰写由国际著名学术出版社Springer以及Elsevier出版的专业书籍章节两篇。
(1) Transport of Monoterpenoid Indole Alkaloids
in Catharanthus rosues”, Plant ABC transporters, In: Signaling and
Communication in Plants, Vol. 22. Geisler, Markus (Ed.), Springer；
(2) Discovery and functional analysis of monoterpenoid
indole alkaloid pathways in plants, In: Methods in ENZYMOLOGY, 515: 207-229,
Elsevier
Email: yufang@dlpu.edu.cn
or fyu0506@gmail.com
办公地址：生物楼532
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