于放，男，教授，博士生导师。辽宁省教育厅生物科学类教指委委员，辽宁省学位委员会学科评议组成员，中国植物生理与植物分子生物学学会植物代谢专业委员会委员，中国野生植物保护协会科技委专家委员，辽宁省植物生理学会常务理事，《植物研究》核心学术期刊编委，《大连工业大学学报》核心学术期刊编委。1974年5月出生。2005年6月毕业于大连理工大学生物化工专业，博士学位。2005至2013年分别在中科院上海植物生理生态研究所、荷兰Leiden University以及加拿大Brock University从事科研工作，2014年3月至大连工业大学工作被评聘为教授。

1. 主要学术研究方向：

(1) 植物次生代谢途径的调控及解析：利用分子生物学及化学手段完成对植物次生代谢物的合成途径相关基因（代谢途径酶基因、转录因子、以及转运基因等）的克隆及鉴定，进而实现对代谢途径的有效调控。

(2) 植物代谢与现代农业：系统研究农业微生物代谢对植物抗性及生长的调节作用，进而揭示发酵产品作为生物农药/农肥调控植物生长的分子机理，为优化从发酵/生物转化法出发进而高效生产农药/农肥提供必需的理论基础。

(3) 生物催化及酶工程：通过基因挖掘及蛋白质工程获得医药、化学品以及天然产物等所需的重要酶，并通过对宿主细胞的驯化及改造，进而利用微生物发酵/生物转化实现对生物制品的高效生产。

2. 招收博士研究生所在学科：轻工技术与工程

3. 招收硕士研究生所在学科：轻工技术与工程；生物学；生物与医药（专硕）

4. 目前承担主要课程：分子生物学；高级细胞生物学

5. 发表论文情况：近年来在Science, PNAS, Current Opinion in Plant Biology, The Plant Journal, Plant Physiology, Tree Physiology等著名学术期刊上发表多篇论文。

近年发表学术论文摘选：

(1) One-pot synthesis and biochemical characterization of the novel magnetic phospholipase D nanoflowers modified with polyphenols. Molecular Catalysis, 2024, DOI: 10.1016/j.mcat.2024.114056

(2) CrJAT1 Regulates Endogenous JA Signaling for Modulating Monoterpenoid Indole Alkaloid Biosynthesis in Catharanthus roseus. Genes, 2024, DOI: 10.3390/genes15030324

(3) Feedback regulation of salicylic acid signaling by isochorismate synthase1 modulates camptothecin biosynthesis in Camptotheca acuminata. Plant Cell, Tissue and Organ Culture, 2024, DOI: 10.1007/s11240-024-02711-x

(4) CaERF1- mediated ABA signal positively regulates camptothecin biosynthesis by activating the iridoid pathway in Camptotheca acuminata. International Journal of Biological Macromolecules, 2024, doi.org/10.1016/j.ijbiomac.2024.129560

(5) Overexpression and truncation of a novel cold-adapted lipase with improved enzymatic characteristics. Protein Expression and Purification, 2024, doi.org/10.1016/j.pep.2023.106376.

(6) Regulation of T16H subcellular localization for promoting its catalytic efficiency in yeast cells. Biotechnology Letters, 2023, doi: 10.1007/s10529-023-03442-3

(7) Effect of the Plant Growth Promoting Rhizobacterium, Cronobacter sp. Y501, for Enhancing Drought Tolerance in Maize (Zea mays L.). Journal of Soil Science and Plant Nutrition, 2023, doi.org/10.1007/s42729-023-01234-2

(8) An ABCG-Type Transporter Facilitates ABA Influx and Regulates Camptothecin Biosynthesis in Camptotheca acuminata. International Journal of Molecular Sciences, 2022, 23(24): 16120

(9) Functional characterization of secologanin synthase-like homologous genes suggests their involvement in the biosynthesis of diverse metabolites in the secoiridoid biosynthetic pathway of Camptotheca acuminata Decne. International Journal of Biological Macromolecules, 2022, 222: 2594-2602

(10) The sensing mechanism of fluorescent probe for PhSH and the process of ESIPT. Photochemical and Photobiological Sciences, 2022, 21: 1055-1065

(11) Citral-loaded chitosan/carboxymethyl cellulose copolymer hydrogel microspheres with improved antimicrobial effects for plant protection. International Journal of Biological Macromolecules, 2020, 164: 986-993

(12) Application of transport engineering to promote catharanthine production in Catharanthus roseus hairy roots. Plant Cell, Tissue and Organ Culture, 2019, 139: 523-530

(13) Two classes of cytochrome P450 reductase genes and their divergent functions in Camptotheca acuminata Decne, International Journal of Biological Macromolecules, 2019, 138: 1098-1108

(14) Microwave assisted extraction of multiple trace levels of intermediate metabolites for camptothecin biosynthesis in Camptotheca acuminata and their simultaneous determination by HPLC-LTQ-orbitrap-MS/MS and HPLC-TSQ-MS, Molecules, 2019, 24(4): 815

(15) A bZIP transcription factor, CaLMF, mediated light-regulated camptothecin biosynthesis in Camptotheca acuminata, Tree Physiology, 2019, 39(3): 372-380

(16) Effects of exogenous salicylic acid on accumulation of camptothecin and gene expression in Camptotheca acuminata, Canadian Journal of Forest Research, 2019, 49(1): 104-110

(17) Improved expression of Bicillus Cereus Phospholipase D in E. coli, ARCHIVOS LATINO AMERICANOS DE NUTRICIóN, 2019, 69: 137-143

(18) Transcriptomics comparison reveals the diversity of ethylene and methyl-jasmonate in roles of TIA metabolism in Catharanthus roseus, BMC genomics, 2018, 19: 508

(19) Identification of a novel phospholipase D gene and effects of carbon sources on its expression in Bacillus cereus ZY12, Journal of Microbiology, 2018, 56: 264-271

(20) Potent and selective inhibition of matrix metalloproteinases by lanthanide trichloride, RSC Advances, 2018, 8: 14347

(21) The ATP binding cassette transporter, VmTPT2/VmABCG1, is involved in export of the monoterpenoid indole alkaloid, vincamine in Vinca minor leaves, Phytochemistry, 2017, 140: 118-124

(22) Antimicrobial activity of saponins produced by two novel endophytic fungi from Panax notoginseng, Natural Product Research, 2017, dx.doi.org/10.1080/14786419.2017.1292265

(23) Cultural endophytic fungi associated with Dendrobium officinale: identification, diversity estimation and their antimicrobial potential, Current Science, 2017, 112: 25

(24) Application of virus-induced gene silencing approach in Camptotheca acuminata, Plant Cell, Tissue and Organ Culture, 2016, 126(3): 533-540

(25) A picrinine-N-methytransferase belongs to a new family of gamma-tocopherol-like methytransferases (gamma-TLMT) found in medicinal plants that make biologically active monoterpenoid indole alkaloids, Plant Physiology，2016, PP-01803

(26) Molecular phylogeny of the Chinese weedy rice based on nrDNA ITS and two cpDNA sequences, INDIAN JOURNAL OF GENETICS AND PLANT BREEDING, 2016, 76: 57-63

(27) Synthesis and characterization of surface-functionalized paramagnetic nanoparticles and their application to immobilization of α-acetolactate decarboxylase, Process Biochemistry, 2015, 50: 1388-1393

(28) Making iridoids/secoiridoids and indole alkaloids: Progress on pathway elucidation, Current Opinion in Plant Biology, 2014, 19: 35-42

(29) 7-Deoxyloganetic acid synthase catalyzes a key 3 step oxidation to form 7-deoxyloganetic acid in Catharanthus roseus iridoid biosynthesis, Phytochemistry, 2014, 101: 23-31

(30) ATP-binding cassette transporter controls leaf surface secretion of anticancer drug components in Catharanthus roseus, Proc. Natl. Acad. Sci. U.S.A. 2013, 110：15830-15835

(31) Functional characterization of amyrin synthase involved in ursolic acid biosynthesis in Catharanthus roseus leaf epidermis, Phytochemistry, 2013, 91： 122-127

(32) Virus induced gene silencing identifies Catharanthus roseus 7-deoxyloganic acid-7-hydroxylase, a step in iridoid and monoterpene indole alkaloid biosynthesis, The Plant Journal, 2013, 76: 754-765

(33) Mining the biodiversity of plants: a revolution in the making, Science, 2012, 336: 1658-1661

6. 承担科研项目情况：主持及参与多项国家及省科研项目，承担的主要科研项目：

(1) 科技部关于科技基础资源调查专项：东北禁伐林区野生经济植物资源调查（2019-2024），子课题，主持；

(2) 国家自然科学基金面上项目：喜树叶表皮细胞中环烯醚萜途径响应水杨酸信号动态调控喜树碱生物合成的分子机制 (2020-2023)，主持；

(3) 辽宁省自然基金资助计划：CaLMF介导光信号调控喜树碱生物合成的分子机制 (2019-2022)，主持；

(4) 国家自然科学基金面上项目：长春花单萜吲哚生物碱生物合成途径中代谢物的胞间转运机制及代谢流调控初探 (2016-2019)，主持；

(5) 辽宁省高等学校创新人才支持计划 (2017-2019)，主持；

(6) 辽宁省教育厅重点领域攻关专项：药用植物次生代谢途径快速解析体系的建立 (2016-2018)，主持；

(7) 辽宁省自然科学基金面上项目：长春碱合成途径代谢流通量调控及中间代谢产物转运基因的克隆 (2015-2016)，主持；

(8) 国家自然科学基金面上项目：共表达分析及内生菌转录组比对结合VIGS技术实现喜树碱合成途径的快速解析 (2017-2020)，参与；

(9) 辽宁省自然科学基金面上项目：不同诱抗模式下ABC Transporter 介导植物根系分泌物外排参与根际微生物差异性应答响应机制 (2018-2020)，参与；

(10) 辽宁省自然科学基金面上项目：转录因子CaHY5在喜树碱生物合成及信号转导中的功能分析 (2015-2016)，参与；

(11) 横向项目：生物酶高效催化生产β-丙氨酸 (2017-2019)，参与。

7. 出版著作情况：撰写由国际著名学术出版社Springer以及Elsevier出版的专业书籍章节两篇。

(1) Transport of Monoterpenoid Indole Alkaloids in Catharanthus rosues”, Plant ABC transporters, In: Signaling and Communication in Plants, Vol. 22. Geisler, Markus (Ed.), Springer；

(2) Discovery and functional analysis of monoterpenoid indole alkaloid pathways in plants, In: Methods in ENZYMOLOGY, 515: 207-229, Elsevier

Email: yufang@dlpu.edu.cn or fyu0506@gmail.com

办公地址：生物楼532